do not (negative auxiliary) / (nonstandard) does not / (African-American Vernacular) Used before an emphatic negative subject.

Stop!, Don't touch that!

Something that must not be done (usually in the phrase dos and don'ts).

(opera) The principal female singer or the leading lady.

(idiomatic) Performed well. / (cooking) Well-cooked. / (specifically, of meat) Cooked through to the center.

An exclamation of praise or endorsement of an achievement.

A voluntary gift or contribution for a specific cause. / The act of giving or bestowing; a grant.

I think what has been said is untrue. / (sarcastic) No, absolutely not (used to refuse or decline something proposed).

One of the Disney cartoon characters, an anthropomorphic white-feathered duck who usually wears a sailor suit and is known for his voice especially in tantrums. / The asteroid 12410.

(Cockney rhyming slang) A fuck.