(American football) An offense in which the quarterback sets up at a distance behind the center and the ball is snapped through the air.

(colloquial) Synonym of shotgun house

(idiomatic, slang) A wedding in which the bride is already pregnant. / (idiomatic) Any similarly reputedly forced partnership, between people or organizations.

(US, idiomatic) To accompany the driver of a vehicle on a journey as an armed escort (originally with a shotgun); (by extension) to accompany someone in order to assist and protect.

(idiomatic) An approach in which the subject is indiscriminate and haphazard, using breadth, spread, or quantity in lieu of accuracy, planning, etc.

(genetics) A DNA sequencing technique in which a large number of small fragments of a long DNA strand are generated at random, sequenced, and reassembled to form a sequence of the original strand.

plural of shotgun pleading

(law) A legal complaint or an answer to a complaint which sets forth an excessive number of facts with no clear organization, and then asserts that those facts describe a cause of action or a defense.

A narrow shack with a door at each end, common in the Southern United States from the end of the Civil War until the 1920s.

(genetics) A laboratory method for the determining the identities of proteins in a mixture.