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(biochemistry) A reagent made from non-fat dry milk, phosphate buffered saline, and sodium azide, which is used to block protein binding sites for laboratory techniques such as blots and the enzyme-linked immunosorbent assay (ELISA).

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/ˈblɒtəʊ/

noun

(biochemistry) A reagent made from non-fat dry milk, phosphate buffered saline, and sodium azide, which is used to block protein binding sites for laboratory techniques such as blots and the enzyme-linked immunosorbent assay (ELISA).

He came home blotto after a night of heavy drinking.

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(biochemistry) A reagent made from non-fat dry milk, phosphate buffered saline, and sodium azide, which is used to block protein binding sites for laboratory techniques such as blots and the enzyme-linked immunosorbent assay (ELISA).

Secondary goat anti-rabbit antiserum coupled to horseradish peroxidase at a dilution of 1:500 in blotto was then incubated with the blot for 1 hour. Again the unbound antibodies were removed by three rinses in 10% blotto for 15 minutes.

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