heminested

In order to allocate alleles with overlapping peaks to a specific locus, the reverse primers used in simple PCRs and the internal reverse primers used in heminested and nested PCRs were 5’ labeled with HEX (4,7,2’,4’,5’,7’-hexachloro-6-carboxyfluorescein), FAM (6-carboxyfluorescein), or TAMRA (6-carboxytetramethylrhodamine), according to the predicted fragment size.