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Stoichiometric release of 6-amidino-2-naphthol allowed highly sensitive fluorometric estimation of active-site concentration in preparations of matriptase and tryptase.
Bottom: Mass spectrum with indicated peaks of hydroxycinnamic acid amides as hordatine precursors (m/z 250–350) and of hordatine A, B, C, and D (D not detected, grey font), hydroxylated hordatines (-OH), and hexose-modified derivates (Hex / 2 Hex) at m/z 550–1000.
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