They also contained restriction enzyme recognition sites and a myctag engineered at their 5' ends to facilitate subsequent synthesis of a plant transformation construct.

She had never been into these crowded streets alone before, and, dazzled by the glittering shop-windows, and confused by the headlong throng and seemingly interminable confusion of their traffic, got herself into continual scrapes by running against the different people she met, receiving such sarcastic admonitions in return as, 'That's your way, my darling,' accompanied by a smart shove from a young gentleman, with a round wooden tray of fresh butter and other cheesemongeries on his head, which she had nearly overturned in the concussion;

This field had had on it about seventy loads of muck per acre, which alone did no good until an application of limesand was given.

Sixteen of these Letters, which were written at the suggestion of the Editor of the “St. James’s Gazette,” appeared in that journal, from which they are now reprinted, by the Editor’s kind permission. They have been somewhat emended, and a few additions have been made.