The root tips were digested in a pectolytic enzyme mixture [0.1% (w/v) pectolyase Y23 (Duchefa, Haarlem, the Netherlands), 0.1% (w/v) cellulase onozuka RS (Duchefa, Haarlem, the Netherlands) and 0.1% (w/v) cytohelicase (Sigma-Aldrich, Steinheim, Germany)] in 10 mM citrate buffer (10mM tri sodium citrate + 10 mM citrate, pH 4.5) at 37 °C for 1 h.

Sir Nathaniel, this Biron is one of the votaries with the king; and here he hath framed a letter to a sequent of the stranger queen's, which accidentally, or by the way of progression, hath miscarried.

We spent many mornings and afternoons eating Long Johns, using the quarters we saved from our bus money.

She stands just a little over 5-foot-6, but her two-handed ground strokes off both wings, inspired by the four-time Australian Open champion Monica Seles, allow her to create sharp angles and counterpunching power.