The serum was separated by centrifugation and stored at -20°C until assayed with the immunofluorometric assay (IFMA) method at the Biomedicine Institute from University of Turku, Finland.

As it nears the sea at Chinkiang, the point at which it makes its entrance into our story, it broadens out into a mile-wide flood, following a serpentine course through flat, marshy country, its waters stained the colour of milk chocolate by its heavy load of alluvial silt and impeded by innumerable islands and sandbanks that vex the way of the navigator. This is the Yangtse—the Yangtse of the low-lying province of Kiangsu, from the ancient walled city of Chinkiang to the open sea—upon whose waters and along whose banks we shall live and move in this story.

Pedro II of Brazil (after dominion)

With these cuts we generally recommend cooking no doner than medium-rare for a juicier product