Jeez, Rae, don't lez out on me.

Here were we, jampacked on a narrow track down which the hathi was certain to come if he came at all , not a fire - iron of any sort among the lot of us, to all appearances not a vestige of an escape on either side

One of these, the Ursal, an inhabitant of the shores of the North Pacific Ocean, […]

The resulting vector containing the SnTox1-Bar knockout construct was PCR-amplified with 5_Tox1Fbar and 3_Tox1Fbar for transformation in toxa3 -10 to result in SnToxA, SnTox1, and SnTox3 deletant mutants.