This problem becomes even more challenging if the study is to be limited to specific cells within a larger population, since other transfection methods, such as viral transfection and lipofection, are not realizable with a high degree of spatial selectivity.Here, we have used a focused femtosecond laser beam to create a small transient hole in the cellular membrane (optoporation) in order to inject nanomolar concentrations of rhodamine phalloidin (an impermeable dye molecule for staining filamentous actin) into targeted living mammalian cells (both HEK and primary cortical neurons).Following optoporation, the bound to the intracellular actin network and rise in fluorescence intensity was observed....

Make sure the workpiece is properly secured in the chuck before turning on the lathe.

A sore matter for a sinner to be corrected, and yet to go light-farrand under it.

My father said that three-leggeds are more detestable than four-leggeds.