A single gradient (part B) usually will suffice, although to obtain maximum purity (e.g., for use as a probe), a velocitization step (part D) and a second CsCl-PI gradient (part E) are added. The velocitization differs from equilibrium gradients in that molecules are pelleted at a rate proportional to their size and largeley independent of conformation.

applied endermically

Wounded and overthrown, the Britons continued their resistance, clung round the legs of the Norman steeds, and cumbered their advance; while their brethren, thrusting with pikes, proved every joint and crevice of the plate and mail, or grappling with the men-at-arms, strove to pull them from their horses by main force, or beat them down with their bills and Welch hooks.

God's good word, both law and gospel, is your bulwark, your defense against bad theology and techniques of spirituality that make you anxious.