All patients underwent fresh ET during the first cycle and did not receive any medication for 3 months prior to IVF/ICSI. Patients were excluded if they had hysteromyoma, adenomyosis, ovarian tumor, endometriosis, polycystic ovarian syndrome (PCOS), hyperprolactinemia, thyroid disease, adrenal disease, diabetes, or a chromosomal aberration(determined by karyotype).