[…] shall a beardless boy, A cocker’d silken wanton, brave our fields […]?

I then gave him a dig on the head with my fist. He squared up, and pouted like an enraged chameleon, looking savagely at me. I gave him another dig, which sent him staggering. He squared again: I gave him another; till at last, as the claret was flowing, he sulked off, and said he would not serve me any more.

She was carried home quite exhausted; the only sign she gave of consciousness was, that when they were about to take her to the room which had formerly been her own, she raised her head, and feebly insisted on being taken to her uncle's. Every thing there was peculiarly his, and there she had gazed, for the last time, on his inanimate features; in that room she could call up his image more distinctly than elsewhere.

Genomic DNA was extracted from parental and mutant cell lines and used as the template to amplify fragments of 200 to 1,500 bp containing the protospacer regions chosen for Cas9 targeting of the TcPFR1, TcPFR2, and TcGP72 genes.