After upwards of Thirty Years Study, and a painful Induſtry, in compiling A New Pandect or Complete Body of the Roman Civil Law; the Firſt Volume of this Undertaking craves Leave to appear in the World under the Patronage and Protection of your Lordſhip, …

The Commission got itself into systematic working order, and for weeks the contributions flowed into its treasury.

Sequential staining is critical as Pyronin Y can also bind DNA and the prestaining of DNA with Hoechst 33342 blocks this.

Note this fine detail in the lower left corner.