Histology sections were cut at 5–6 µm on a rotary microtome, mounted on glass slides, and stained with Harris’s haematoxylin and eosin in a Stainomatic specimen stainer.

… marke his colour and his shape, that is to say, a comely well proportioned head, with an outlooking eye, good well raised shoulders, and a thicke large breast …

Choanoflagellates and filasterea are considered the closest unicellular organisms to metazoan animals.

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