最終更新日:2022/12/24
Removed ovary lobes were divided into small sections and treated with 2 mg/mL Collagenase A (Boehringer Mannheim, Indianapolis, IN, USA) in OR2 buffer for at least an hour to defolliculate the oocytes.
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Removed ovary lobes were divided into small sections and treated with 2 mg/mL Collagenase A (Boehringer Mannheim, Indianapolis, IN, USA) in OR2 buffer for at least an hour to defolliculate the oocytes.