最終更新日：2022/12/24
In order to allocate alleles with overlapping peaks to a specific locus, the reverse primers used in simple PCRs and the internal reverse primers used in heminested and nested PCRs were 5’ labeled with HEX (4,7,2’,4’,5’,7’-hexachloro-6-carboxyfluorescein), FAM (6-carboxyfluorescein), or TAMRA (6-carboxytetramethylrhodamine), according to the predicted fragment size.
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元となった例文
In
order
to
allocate
alleles
with
overlapping
peaks
to
a
specific
locus,
the
reverse
primers
used
in
simple
PCRs
and
the
internal
reverse
primers
used
in
heminested
and
nested
PCRs
were
5’
labeled
with
HEX
(4,7,2’,4’,5’,7’-hexachloro-6-carboxyfluorescein),
FAM
(6-carboxyfluorescein),
or
TAMRA
(6-carboxytetramethylrhodamine),
according
to
the
predicted
fragment
size.
