最終更新日:2022/12/24
100 μl reactions were carried out containing equal volumes of buffered substrate (pH 4.5) and suitably diluted culture filtrate which were then incubated at 40°C for 1 h in the case of the endoarabinanase, endo-1,4-β-glucanase and endo-1,4-β-xylanase activities and 16 h for the endo-1,4-β-galactanase and rhamnogalacturonan activities.
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100
μl
reactions
were
carried
out
containing
equal
volumes
of
buffered
substrate
(pH
4.5)
and
suitably
diluted
culture
filtrate
which
were
then
incubated
at
40°C
for
1
h
in
the
case
of
the
endoarabinanase,
endo-1,4-β-glucanase
and
endo-1,4-β-xylanase
activities
and
16
h
for
the
endo-1,4-β-galactanase
and
rhamnogalacturonan
activities.