Coordinate term: Tejano
One hour prior to transfection, the culture medium was replaced with serum-free RPMI medium. 0.4 μg of DNA (pNN9 or DNA ministring), diluted in 50 μl of serum-free OptiMEM culture medium, was mixed with different aliquots of 1.5 mM 16-3-16 gemini surfactant solution to yield N + /P − charge ratios of 3:1 and 5:1.
Stanley had been with me for only a few weeks when he vacuumed up his food even faster than normal, came back into the living room, tottered, and keeled over, eyes closed, the tip of his tongue protruding pinkly through his teeth.
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