He liked to wear an old ragtag coat that was so threadbare that he'd get sunburned through it.
Waiter! There's a fly in my soup!
Briefly, various concentrations of austrobailignan-1 were added to the topoisomerase-1 activity reaction mixture (including 250 ng pHOT-1 plasmid DNA, 0.3 U calf thymus DNA topoisomerase 1, 20 mM Tris-HCl, pH 8.0, 72 mM KCl, 5 mM MgCl 2, 5 mM dithiothreitol, 2 mM spermidine, 0.01% bovine serum albumin) for 30 min at 37°C, and the reaction was stopped by loading dye solution (2.5% SDS, 25 mM EDTA, pH 8.0, 15% ficoll-400, 0.05% bromophenol blue, and 0.05% xylene cyanole).
A European custom had young women prepare cockle-bread, a food intended to excite men's passion, by sitting on dough and wiggling around to knead it, sometimes reciting a rhyme in the process (Up with my heels and down with my head/And this is the way to mould cockle-bread is an example).
cockle-bread,
Up with my heels and down with my head/And this is the way to mould cockle-bread
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