As controls, we used samples previously treated with 100 mM ditiotreitol (DTT) for 30 min at 30 °C before precipitation of the proteins with TCA.
And to be sure, as your ladyship says, the girl hath always appeared like a good, honest, plain girl, and not vain of her face, forsooth, as some wanton husseys in the neighbourhood are.
But as we allow not altogether that ſevere education of the old Spartanes in their children, hazarding many times the healths of young and tender bodies, by ſome tedious ague; yea, alſo their liues, by the miſchance of a leape or ſtumbling of their horſe: ſo as much doe I deteſt that effœminacy of the moſt, that burne out day and night in their beds, and by the fire ſide; in trifles, gaming, or courting their yellow Miſtreſſes all the Winter in a City; appearing but as Cuckoes in the Spring, one time in the yeere to the Countrey and their tenants, leaving the care of keeping good houſes at Chriſtmas, to the honeſt Yeomen of the Countrey.
Proses are parts of the Office of the Mass which are sung just before the Gospel, upon great Festivals. The French also call those Rhythmical Hymns Proses, which are sung in their Offices in the Church of Rome, in which Rhime only, and not Quantity of Syllables, is observed.
